The applied antibiotic or other substances for curing mastitis, although cure the illness but any of them might be left in milk and cause some problems for dairy products suppliers,so fast detection of antibiotic is too important.
The most important groups of antibiotics are Betalactoms and Tetrasyclyns which used to cure mastitis.
The harmful effects of using milk included antibiotic are as follow: 

• Industrial effects :prevention of starters grouth and producing acid in fermented products , so these cause problems in production.

• Clinical effects: using milk included anti biotic cause alergetic reflexs.

• Changes in intestine microbic flora